Active Recombinant Human NEDD4 Protein (Leu570-Asp1319), N-6×His-tagged

• Cat.No.: NEDD4-14H
• Product Overview: Recombinant Human His6-NEDD4 Protein (Leu570-Asp1319) with a N-terminal Met and 6-His tag was expressed in Trichoplusia ni (baculovirus).

Specification

• Description: This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.
• Source: Insect cells
• Species: Human
• Tag: N-6×His
• Bio-activity: Recombinant Human NEDD 4 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application.
• Molecular Mass: 88 kDa
• Protein Length: Leu570-Asp1319
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and EDTA.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: NEDD4 neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase [ Homo sapiens (human) ]
• Official Symbol: NEDD4
• Synonyms: NEDD4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; neural precursor cell expressed, developmentally down regulated 4; E3 ubiquitin-protein ligase NEDD4; KIAA0093; MGC176705; NEDD4 1; receptor potentiating factor 1; RPF1; NEDD-4; receptor-potentiating factor 1; cell proliferation-inducing gene 53 protein; neural precursor cell expressed developmentally down-regulated protein 4; NEDD4-1
• Gene ID: 4734
• mRNA Refseq: NM_006154
• Protein Refseq: NP_006145
• MIM: 602278
• UniProt ID: P46934