| Description : |
NEK1 (NIMA-related kinase 1) is a serine/threonine protein kinase that is involved in a variety of cellular processes, including DNA damage response, cell cycle progression, and cilia function. NEK1 has been shown to be involved in the regulation of DNA damage response, including repair of double-strand breaks, and mutations in NEK1 have been linked to several diseases, including cancer and neurodegenerative disorders. Research has suggested that NEK1 plays a critical role in maintaining the integrity of the primary cilium, which is a sensory organelle present on the surface of most mammalian cells. Dysfunction of primary cilia has been linked to a wide range of developmental and disease-related conditions, including polycystic kidney disease and retinal degeneration. NEK1 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST,His |
| Protein length : |
1-505 aa |
| Form : |
0.86 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
637 U/mg, where one unit of NEK1 activity is defined as 1 nmol phosphate incorporated into 250 μM RLGRDKYKTLRQIRQ per minute at 30 centigrade with a final ATP concentration of 100 μM. |
| Molecular Mass : |
86 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSQTSL YKKAGTMEKY 241 VRLQKIGEGS FGKAILVKST EDGRQYVIKE INISRMSSKE REESRREVAV LANMKHPNIV 301 QYRESFEENG SLYIVMDYCE GGDLFKRINA QKGVLFQEDQ ILDWFVQICL ALKHVHDRKI 361 LHRDIKSQNI FLTKDGTVQL GDFGIARVLN STVELARTCI GTPYYLSPEI CENKPYNNKS 421 DIWALGCVLY ELCTLKHAFE AGSMKNLVLK IISGSFPPVS LHYSYDLRSL VSQLFKRNPR 481 DRPSVNSILE KGFIAKRIEK FLSPQLIAEE FCLKTFSKFG SQPIPAKRPA SGQNSISVMP 541 AQKITKPAAK YGIPLAYKKY GDKKLHEKKP LQKHKQAHQT PEKRVNTGEE RRKISEEAAR 601 KRRLEFIEKE KKQKDQIISL MKAEQMKRQE KERLERINRA REQGWRNVLS AGGSGEVKAP 661 FLGSGGTIAP SSFSSRGQYE HYHAIFDQMQ QQRAEDNEAK WKREIYGRGL PERGILPGVR 721 PGFPYGAAGH HHFPDADDIR KGPGPGHHHH HH |
| Purity : |
Purity 47% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
