Active Recombinant Human NEK7(2-end) Protein, N-His-tagged

• Cat.No.: NEK7-174H
• Product Overview: N-terminal 6Histagged, recombinant, human NEK7, amino acids 2–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: NEK7 is a protein kinase which plays an important role in mitotic cell cycle progression and required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. It phosphorylates RPS6KB1 (by similarity). It phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression. NEK7 is an essential protein for NLRP3 inflammasome assembly and activation that acts downstream stimuli such as K+ efflux. Dispensable for the induction of core inflammasome components, but necessary for subsequent formation of the NLRP3:PYCARD complex, and activation of CASP1. It serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division. It exerts its effects on the NLRP3 inflammasome independently of its kinase activity.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 2.213mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 16U/mg, where one unit of NEK7 activity is defined as 1nmol phosphate incorporated into 300μM (FLAKSFGSPNRAYKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 37.9 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSDE QSQGMQGPPV PQFQPQKALR PDMGYNTLAN 61 FRIEKKIGRG QFSEVYRAAC LLDGVPVALK KVQIFDLMDA KARADCIKEI DLLKQLNHPN 121 VIKYYASFIE DNELNIVLEL ADAGDLSRMI KHFKKQKRLI PERTVWKYFV QLCSALEHMH 181 SRRVMHRDIK PANVFITATG VVKLGDLGLG RFFSSKTTAA HSLVGTPYYM SPERIHENGY 241 NFKSDIWSLG CLLYEMAALQ SPFYGDKMNL YSLCKKIEQC DYPPLPSDHY SEELRQLVNM 301 CINPDPEKRP DVTYVYDVAK RMHACTASS
• Purity: Purity 95.2% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of

Gene Information

• Gene Name: NEK7 NIMA related kinase 7 [ Homo sapiens (human) ]
• Official Symbol: NEK7
• Synonyms: NEK7; NIMA related kinase 7
• Gene ID: 140609
• mRNA Refseq: NM_133494.3
• Protein Refseq: NP_598001.1
• MIM: 606848
• UniProt ID: Q8TDX7