| Description : |
NEK9 (NIMA-related kinase 9) is a serine/threonine protein kinase that belongs to the NEK (never in mitosis gene A-related kinase) family of kinases. It is involved in a variety of cellular processes, including regulation of the cell cycle, mitosis, and the DNA damage response. NEK9 is expressed in a wide range of tissues and cell types, and is regulated by various post-translational modifications, including phosphorylation and ubiquitination. Research has suggested that NEK9 plays a role in several diseases, including cancer and ciliopathies. In cancer, NEK9 has been shown to promote cell proliferation and survival, and inhibition of NEK9 has been suggested as a potential cancer therapy. In ciliopathies, NEK9 has been implicated in the regulation of cilia length and number, and mutations in NEK9 have been associated with ciliopathy-related disorders such as Joubert syndrome. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
1-324 aa |
| Form : |
2.168mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
6027U/mg, where one unit of NEK9, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml MBP per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
41 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQMSVL GEYERHCDSI NSDFGSESGG 61 CGDSSPGPSA SQGPRAGGGA AEQEELHYIP IRVLGRGAFG EATLYRRTED DSLVVWKEVD 121 LTRLSEKERR DALNEIVILA LLQHDNIIAY YNHFMDNTTL LIELEYCNGG NLYDKILRQK 181 DKLFEEEMVV WYLFQIVSAV SCIHKAGILH RDIKTLNIFL TKANLIKLGD YGLAKKLNSE 241 YSMAETLVGT PYYMSPELCQ GVKYNFKSDI WAVGCVIFEL LTLKRTFDAT NPLNLCVKIV 301 QGIRAMEVDS SQYSLELIQM VHSCLDQDPE QRPTADELLD RPLLRKRRRE MEEKVTLLNA |
| Purity : |
Purity 89.7% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
