Active Recombinant Human NRP1 protein, Fc-tagged

• Cat.No.: NRP1-0566H
• Product Overview: Recombinant Human NRP1 protein(Phe22-Lys644), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human VEGF165, premium grade at 2 μg/mL (100 μL/well) can bind Human NRP1, Fc Tag with a linear range of 0.2-3 ng/mL. Immobilized Human NRP1, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, His,Avitag with a linear range of 0.008-0.25 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 96.5 kDa. The protein migrates as 105-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe22-Lys644
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRP1 neuropilin 1 [ Homo sapiens ]
• Official Symbol: NRP1
• Synonyms: NRP1; neuropilin 1; neuropilin-1; CD304; NRP; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; NP1; BDCA4; DKFZp781F1414; DKFZp686A03134
• Gene ID: 8829
• mRNA Refseq: NM_001024628
• Protein Refseq: NP_001019799
• MIM: 602069
• UniProt ID: O14786