Active Recombinant Human NTRK3(510-end) Protein, N-GST,C-His-tagged

• Cat.No.: NTRK3-250H
• Product Overview: N-terminal GST-tagged and C-terminal 6His-tagged, recombinant, human TrkC amino acids 510-end, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose followed by gel filtration

Specification

• Description: TrkC is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: 510-end
• Form: 0.308mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 998U/mg, where one unit of TrkC, active activity is defined as 1nmol phosphate incorporated into 500μM (GEEPLYWSFPAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 64 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFM VIENPQYFRQ 241 GHNCHKPDTY VQHIKRRDIV LKRELGEGAF GKVFLAECYN LSPTKDKMLV AVKALKDPTL 301 AARKDFQREA ELLTNLQHEH IVKFYGVCGD GDPLIMVFEY MKHGDLNKFL RAHGPDAMIL 361 VDGQPRQAKG ELGLSQMLHI ASQIASGMVY LASQHFVHRD LATRNCLVGA NLLVKIGDFG 421 MSRDVYSTDY YRVGGHTMLP IRWMPPESIM YRKFTTESDV WSFGVILWEI FTYGKQPWFQ 481 LSNTEVIECI TQGRVLERPR VCPKEVYDVM LGCWQREPQQ RLNIKEIYKI LHALGKATPI 541 YLDILGHHHH HH
• Purity: Purity 95% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: NTRK3 neurotrophic receptor tyrosine kinase 3 [ Homo sapiens (human) ]
• Official Symbol: NTRK3
• Synonyms: NTRK3; neurotrophic receptor tyrosine kinase 3; TRKC; GP145-TrkC; gp145(trkC)
• Gene ID: 4916
• mRNA Refseq: NM_001007156.3
• Protein Refseq: NP_001007157.1
• MIM: 191316
• UniProt ID: Q16288