Active Recombinant Human PASK(995-end) Protein, N-His-tagged

• Cat.No.: PASK-183H
• Product Overview: N-terminal 6Histagged, recombinant, human PASK, amino acids 995–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: PASK kinase is a serine/threonine-protein kinase is involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. It probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 995-end
• Form: 0.748mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1211U/mg, where one unit of PASK, active activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 41.5 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQYSQK YSTMSPLGSG AFGFVWTAVD 61 KEKNKEVVVK FIKKEKVLED CWIEDPKLGK VTLEIAILSR VEHANIIKVL DIFENQGFFQ 121 LVMEKHGSGL DLFAFIDRHP RLDEPLASYI FRQLVSAVGY LRLKDIIHRD IKDENIVIAE 181 DFTIKLIDFG SAAYLERGKL FYTFCGTIEY CAPEVLMGNP YRGPELEMWS LGVTLYTLVF 241 EENPFCELEE TVEAAIHPPY LVSKELMSLV SGLLQPVPER RTTLEKLVTD PWVTQPVNLA 301 DYTWEEVFRV NKPESGVLSA ASLEMGNRSL SDVAQAQELC GGPVPGEAPN GQGCLHPGDP 361 RLLTS
• Purity: Purity 60.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PASK PAS domain containing serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: PASK
• Synonyms: PASK; PAS domain containing serine/threonine kinase; STK37; PASKIN
• Gene ID: 23178
• mRNA Refseq: NM_015148.4
• Protein Refseq: NP_055963.2
• MIM: 607505
• UniProt ID: Q96RG2