Active Recombinant Human PDCD1LG2 protein, Fc-tagged, Biotinylated

• Cat.No.: PDCD1LG2-051H
• Product Overview: Biotinylated Recombinant Human PDCD1LG2(Leu20-Pro219) protein, fused to Fc tag at the C-terminus, was expressed in NS0 cells .

Specification

• Description: PDCD1LG2, also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature human PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. Alternative splicing generates additional isoforms that lack the second Ig-like domain and may be substituted and truncated following the first Ig-like domain (4). PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3, 5, 6). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (7-11). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 12, 13). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (9, 11).
• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Leu20
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human PDCD1 Fc Chimera is immobilized at 100 ng/mL, 100 μL/well, the concentration of Biotinylated Recombinant PDCD1G2 Fc Chimera that produces 50% of the optimal binding response is 0.04-0.24 μg/mL.
• Protein length: Leu20-Pro219
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 250 μg/mL in PBS.

Gene Information

• Gene Name: PDCD1LG2 programmed cell death 1 ligand 2 [ Homo sapiens ]
• Official Symbol: PDCD1LG2
• Synonyms: PDCD1LG2; programmed cell death 1 ligand 2; B7 dendritic cell molecule; B7 DC; bA574F11.2; Btdc; CD273; PD L2; PDL2; B7-DC; PD-1 ligand 2; PD-1-ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; programmed death ligand 2; B7DC; PD-L2; PDCD1L2; MGC142238; MGC142240
• Gene ID: 80380
• mRNA Refseq: NM_025239
• Protein Refseq: NP_079515
• MIM: 605723
• UniProt ID: Q9BQ51