Active Recombinant Human PDGFB Protein

• Cat.No.: PDGFB-45H
• Product Overview: Recombinant Human PDGFB Protein without tag was expressed in E. coli.

Specification

• Description: PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-aa, ab and bb are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. The PDGFs plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. They are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development.
• Source: E. coli
• Species: Human
• Bio-activity: The activity was measured by its ability to stimulate the proliferation of 3T3 mouse fibroblast cells.
• Molecular Mass: 12.3 kDa (monomer)
• Endotoxin: <1 EU/ μg
• Purity: >95% by SDS-PAGE
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -80 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in 10mM acetic acid without carrier protein
• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 10mM acetic acid containing 0.1% endotoxin-free recombinant human serum albumin.

Gene Information

• Gene Name: PDGFB platelet derived growth factor subunit B [ Homo sapiens (human) ]
• Official Symbol: PDGFB
• Synonyms: PDGFB; platelet derived growth factor subunit B; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; platelet-derived growth factor subunit B; PDGF subunit B; PDGF, B chain; becaplermin; epididymis secretory sperm binding protein; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor, beta polypeptide (oncogene SIS); proto-oncogene c-Sis
• Gene ID: 5155
• mRNA Refseq: XM_047441394
• Protein Refseq: XP_047297350
• MIM: 190040
• UniProt ID: P01127