Active Recombinant Human Phosphatase And Tensin Homolog

• Cat.No.: PTEN-1094H

Specification

• Cat. No.: PTEN-1094H
• Description: PTEN is one of the most frequently mutated tumor suppressors in human cancer. PTEN is a dual-specificity phosphatase with both protein phosphatase and lipid phosphatase activity. It is the first phosphatase identified as a tumor suppressor. It may be involved in almost all types of cancer, both solid tumors and hematological malignancies. The best described substrate of PTEN is phosphatidylinositol (3,4,5)-tris-phosphate (PtdIns (3,4,5) P3. PTEN removes the phosphate in PtdIns (3,4,5) P3 to generate PtdIns (4,5) P2 and serves to counter-balance the effects of PI3 Kinase, which normally generate PtdIns (3,4,5) P3). PTEN regulate the signaling pathway of PI3 Kinases by preventing localization of proteins with pleckstrin homology domains to the cell membrane.
• Source: Sf21 cells.
• Application: 20 μM substrate (FDP), 50 mM imidazole pH 7.2, 200 μM EGTA, 0.02% mercaptoethanol, 5 mM MgCl2 and 0.1 mg/ml BSA at 37℃.
• Usage: For in vitro use only.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Activity: 1 μg of recombinant human PTEN hydrolyzes a fluorogenic substrate (fluorescein diphosphate) releasing fluorescence (excitation/emission 490/514 nm) at 10 AFU/min.
• Purity: > 95% by SDS-PAGE.
• Storage: Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: PTEN phosphatase and tensin homolog [ Homo sapiens ]
• Synonyms: PTEN; BZS; MGC11227; ADSF; FIZZ3; HXCP1; RSTN; XCP1; resistin; MHAM; MMAC1; PTEN1; TEP1; MGC126603; MGC126609; phosphatase and tensin homolog; Adipose tissue-specific secretory factor; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; found in inflammatory zone 3; Phosphatase and tensin homolog (mutated in multiple advanced cancers 1)
• Gene ID: 5728
• mRNA Refseq: NM_000314
• Protein Refseq: NP_000305
• MIM: 601728
• UniProt ID: P60484
• Chromosome Location: 10q23
• Pathway: Endometrial cancer; Focal adhesion; Prostate cancer; Glioma; Inositol phosphate metabolism; Melanoma; Phosphatidylinositol signaling system; Small cell lung cancer; Tight junction; p53 signaling pathway; Pathways in cancer
• Function: PDZ domain binding; hydrolase activity; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein serine/threonine phosphatase activity; platelet-derived growth factor receptor binding; phosphatidylinositol-3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity

Reviews

11/05/2022

Gel electrophoresis consistently displayed well-defined bands, affirming the product's purity and reliability.

01/18/2021

The insights gained from the product's application justified its cost, marking a prudent investment in our research endeavors.

12/20/2020

Comprehensive documentation streamlined the integration of the product into our experimental workflow.

Q&As

Apart from its lipid phosphatase activity, what additional function has been attributed to PTEN?

In addition to its lipid phosphatase activity, PTEN has an additional function known as a scaffolding protein that contributes to its tumor-suppressive role.

In which cellular compartment does PTEN primarily exert its lipid phosphatase function?

PTEN primarily exerts its lipid phosphatase function in the cytoplasm, where it regulates the PI3K/AKT pathway.

What is the significance of the FBXO22-mediated degradation of nuclear PTEN in promoting tumorigenesis?

The significance of FBXO22-mediated degradation of nuclear PTEN in promoting tumorigenesis involves the loss of PTEN's tumor-suppressive functions, leading to uncontrolled cell growth.

How is the expression of nuclear PTEN (核PTEN) regulated, especially in the context of tumorigenesis?

The expression of nuclear PTEN (核PTEN) is regulated, especially in the context of tumorigenesis, through mechanisms involving post-translational modifications and subcellular localization.

What differences are observed in the expression patterns of nuclear PTEN and cytoplasmic PTEN (浆PTEN) in various cancer processes?

Differences in expression patterns of nuclear PTEN and cytoplasmic PTEN in various cancer processes include alterations in nuclear PTEN levels contributing to tumorigenesis.

What is the classical function of the PTEN protein encoded by the PTEN gene?

The classical function of the PTEN protein is to regulate cell growth and prevent tumor formation by suppressing the PI3K/AKT signaling pathway.

What role does nuclear PTEN play in mediating the non-phosphatase-dependent function?

Nuclear PTEN plays a role in mediating the non-phosphatase-dependent function related to genomic stability and DNA repair.