Active Recombinant Human PI4K2B, GST-tagged

• Cat.No.: PI4K2B-1076H
• Product Overview: Full-length recombinant human PI4K2B was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4,5-bisphosphate synthesis.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 218 nmol/min/mg
• Molecular Mass: ~84 kDa
• Protein length: Full Length
• Purity: >90%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PI4K2B phosphatidylinositol 4-kinase type 2 beta [ Homo sapiens ]
• Official Symbol: PI4K2B
• Synonyms: PI4K2B; phosphatidylinositol 4-kinase type 2 beta; phosphatidylinositol 4-kinase type 2-beta; FLJ11105; PI4KIIB; PIK42B; PI4KII-BETA; phosphatidylinositol 4-kinase type II-beta; phosphatidylinositol 4-kinase type-II beta; NP_060793.2; EC 2.7.1.67
• Gene ID: 55300
• mRNA Refseq: NM_018323
• Protein Refseq: NP_060793
• MIM: 612101
• UniProt ID: Q8TCG2
• Chromosome Location: 4p15.2
• Pathway: 3-phosphoinositide biosynthesis; D-myo-inositol (1,4,5)-trisphosphate biosynthesis; Inositol phosphate metabolism
• Function: 1-phosphatidylinositol 4-kinase activity; ATP binding