Active Recombinant Human PLA2G1B Protein, His-tagged

• Cat.No.: PLA2G1B-254H
• Product Overview: Recombinant human PLA2G1B (NP_000919.1) (Met 1-Ser 148) with a C-terminal polyhistidine tag was expressed.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Asp 16
• Form: Lyophilized from sterile 10mM Tris, 5mM CaCl2, pH 8.0, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphomethanol. The specific activity is >2,500 pmol/min/μg.
• Molecular Mass: The recombinant human PLA2G1B comprises 144 amino acids after removal of the signal peptide, and has a predicted molecular mass of 16.2 kDa. rh PLA2G1B migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PLA2G1B phospholipase A2, group IB (pancreas) [ Homo sapiens ]
• Official Symbol: PLA2G1B
• Synonyms: PLA2G1B; phospholipase A2, group IB (pancreas); PLA2, PLA2A, PPLA2; phospholipase A2; group IB phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; PLA2; PLA2A; PPLA2; MGC119834; MGC119835
• Gene ID: 5319
• mRNA Refseq: NM_000928
• Protein Refseq: NP_000919
• MIM: 172410
• UniProt ID: P04054
• Chromosome Location: 12q23-qter
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem