Active Recombinant Human PLA2G2A Protein (1-124; variant N1A)

• Cat.No.: PLA2G2A-12H
• Product Overview: Active recombinant human sPLA2 (Type IIA) protein (1-124; variant N1A) without tag was expressed in E. coli

Specification

• Description: The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: Tag Free
• Molecular Mass: 13.9 kDa
• Protein Length: 1-124; variant N1A
• Purity: ≥ 85 % estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze 1 nmol of diheptanoyl thio-PC per minute at 37 centigrade in 25 mM Tris-HCl, pH 7.5, containing 10 mMcalcium chloride, 100 mM potassium chloride, 0.3 mM Triton X-100, 0.44 mM DTNB, and 1.48 mM diheptanoyl thio-PC.
• Stability: ≥ 1 year
• Storage: At -80 centigrade
• Storage Buffer: 50 mM Tris-HCl, pH 7.6, with 100 mM sodium chloride and 10 mM calcium chloride.
• Shipping: Dry ice in continental US; may vary elsewhere

Gene Information

• Gene Name: PLA2G2A phospholipase A2 group IIA [ Homo sapiens (human) ]
• Official Symbol: PLA2G2A
• Synonyms: PLA2G2A; phospholipase A2 group IIA; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; phospholipase A2, membrane associated; GIIC sPLA2; NPS-PLA2; group IIA phospholipase A2; non-pancreatic secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); EC 3.1.1.4
• Gene ID: 5320
• mRNA Refseq: NM_000300
• Protein Refseq: NP_000291
• MIM: 172411
• UniProt ID: P14555