| Description : |
Polo-like kinases (PLKs) are a family of serine/threonine kinases related to the polo gene product of Drosophila melanogaster, originally identified from a mitotic mutant that displayed abnormal spindle poles. PLKs play essential roles in several stages of mitosis and cytokinesis with a dynamic pattern of localization to centrosomes, kinetochores and central spindle structures during the cell cycle. PLK1 has been shown to be overexpressed in many human tumors, such as non-small cell lung cancer, squamous cell carcinomas of the head and neck, melanomas, oropharyngeal carcinomas, and ovarian and endometrial carcinomas. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Form : |
2.301mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
60U/mg, where one unit of Plk1 activity is defined as 1nmol phosphate incorporated into 1.25mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
69.3 kDa |
| AA Sequence : |
1 MHHHHHHMSA AVTAGKLARA PADPGKAGVP GVAAPGAPAA APPAKEIPEV LVDPRSRRRY 61 VRGRFLGKGG FAKCFEISDA DTKEVFAGKI VPKSLLLKPH QREKMSMEIS IHRSLAHQHV 121 VGFHGFFEDN DFVFVVLELC RRRSLLELHK RRKALTEPEA RYYLRQIVLG CQYLHRNRVI 181 HRDLKLGNLF LNEDLEVKIG DFGLATKVEY DGERKKTLCG TPNYIAPEVL SKKGHSFEVD 241 VWSIGCIMYT LLVGKPPFET SCLKETYLRI KKNEYSIPKH INPVAASLIQ KMLQTDPTAR 301 PTINELLNDE FFTSGYIPAR LPITCLTIPP RFSIAPSSLD PSNRKPLTVL NKGLENPLPE 361 RPREKEEPVV RETGEVVDCH LSDMLQQLHS VNASKPSERG LVRQEEAEDP ACIPIFWVSK 421 WVDYSDKYGL GYQLCDNSVG VLFNDSTRLI LYNDGDSLQY IERDGTESYL TVSSHPNSLM 481 KKITLLKYFR NYMSEHLLKA GANITPREGD ELARLPYLRT WFRTRSAIIL HLSNGSVQIN 541 FFQDHTKLIL CPLMAAVTYI DEKRDFRTYR LSLLEEYGCC KELASRLRYA RTMVDKLLSS 601 RSASNRLKAS |
| Purity : |
Purity 91.7% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
