Active Recombinant Human PRKAA1 Protein, His-tagged
Cat.No. : | PRKAA1-007H |
Product Overview : | Active recombinant human C-terminal His-tagged AMPK complex (α1: 1-559; β1: 1-270; γ1: 1-331) was expressed in insect cells. |
- Specification
- Gene Information
- Related Products
Description : | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Source : | Insect cell |
Species : | Human |
Tag : | His |
Bio-activity : | Assay performed in Tris-buffered solution containing Mg2+ with 1 mM DTT using AMARA peptide as a substrate (0.1 mg/mL), 20μM ATP and 100μM AMP. Reaction was done at 30 centigrade for 45 min. Amount of ATP transferred was calculated using via luminescent assay. |
Molecular Mass : | ~146 kDa (α1: 65 kDa; β1: 38 kDa; γ1: 31 kDa) |
Protein length : | α1: 1-559; β1: 1-270; γ1: 1-331 |
Purity : | ≥90% as determined by SDS-PAGE |
Stability : | ≥ 2 years |
Storage : | At -80 centigrade. |
Storage Buffer : | 50 mM sodium phosphate, pH 7.0, with 300 mM sodium chloride, 150 mM imidazole, 0.25 mM DTT, and 25% glycerol |
Gene Name : | PRKAA1 protein kinase AMP-activated catalytic subunit alpha 1 [ Homo sapiens (human) ] |
Official Symbol : | PRKAA1 |
Synonyms : | PRKAA1; protein kinase AMP-activated catalytic subunit alpha 1; AMPK; AMPKa1; AMPK alpha 1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK subunit alpha-1; AMPK, alpha, 1; HMGCR kinase; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; tau-protein kinase PRKAA1; EC 2.7.11.1; EC 2.7.11.26; EC 2.7.11.27; EC 2.7.11.31 |
Gene ID : | 5562 |
mRNA Refseq : | NM_206907 |
Protein Refseq : | NP_996790 |
MIM : | 602739 |
UniProt ID : | Q13131 |
Products Types
◆ Recombinant Protein | ||
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PRKAA1-2953H | Recombinant Human PRKAA1 Protein, MYC/DDK-tagged | +Inquiry |
PRKAA1-01H | Recombinant Human PRKAA1 Protein (K45R) | +Inquiry |
PRKAA1-147H | Recombinant Human PRKAA1 Protein, His-tagged | +Inquiry |
PRKAA1-06H | Recombinant Human PRKAA1 complex Protein, N-Flag-tagged | +Inquiry |
◆ Lysates | ||
PRKAA1-1412HCL | Recombinant Human PRKAA1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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