Active Recombinant Human PRKCH(2-end) Protein, N-His-tagged

• Cat.No.: PRKCH-196H
• Product Overview: N-terminal 6Histagged, recombinant human PKC eta, amino acids 2–end expressed by baculovirus in SF21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Protein kinase C h (PKCh) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.154mg/ml of enzyme in 20mM Tris/HCl pH7.5, 10mM benzamidine, 5% glycerol, 1mM EDTA, 1mM EGTA, 1mM PMSF, 0.02% Triton X-100, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 530U/mg, where one unit of PKC eta, active activity is defined as 1nmol phosphate incorporated into 50μM PKC tide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 78.6 kDa
• AA Sequence: 1 MHHHHHHSSG TMKFNGYLRV RIGEAVGLQP TRWSLRHSLF KKGHQLLDPY LTVSVDQVRV 61 GQTSTKQKTN KPTYNEEFCA NVTDGGHLEL AVFHETPLGY DFVANCTLQF QELLRTTGAS 121 DTFEGWVDLE PEGKVFVVIT LTGSFTEATL QRDRIFKHFT RKRQRAMRRR VHQINGHKFM 181 ATYLRQPTYC SHCREFIWGV FGKQGYQCQV CTCVVHKRCH HLIVTACTCQ NNINKVDSKI 241 AEQRFGINIP HKFSIHNYKV PTFCDHCGSL LWGIMRQGLQ CKICKMNVHI RCQANVAPNC 301 GVNAVELAKT LAGMGLQPGN ISPTSKLVSR STLRRQGKES SKEGNGIGVN SSNRLGIDNF 361 EFIRVLGKGS FGKVMLARVK ETGDLYAVKV LKKDVILQDD DVECTMTEKR ILSLARNHPF 421 LTQLFCCFQT PDRLFFVMEF VNGGDLMFHI QKSRRFDEAR ARFYAAEIIS ALMFLHDKGI 481 IYRDLKLDNV LLDHEGHCKL ADFGMCKEGI CNGVTTATFC GTPDYIAPEI LQEMLYGPAV 541 DWWAMGVLLY EMLCGHAPFE AENEDDLFEA ILNDEVVYPT WLHEDATGIL KSFMTKNPTM 601 RLGSLTQGGE HAILRHPFFK EIDWAQLNHR QIEPPFRPRI KSREDVSNFD PDFIKEEPVL 661 TPIDEGHLPM INQDEFRNFS YVSPELQP
• Purity: Purity 80.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKCH protein kinase C eta [ Homo sapiens (human) ]
• Official Symbol: PRKCH
• Synonyms: PRKCH; protein kinase C eta; PKCL; PKC-L; PRKCL; uORF2; nPKC-eta
• Gene ID: 5583
• mRNA Refseq: NM_006255.5
• Protein Refseq: NP_006246.2
• MIM: 605437
• UniProt ID: P24723