| Description : |
CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
598-end |
| Form : |
0.829mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 10% glycerol, 0.03% Brij-35, 0.1mM EGTA, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
31108U/mg, where one unit of CD45, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4- methylumbelliferyl phosphate (DiFMUP) at room temperature. |
| Molecular Mass : |
84 kDa |
| AA Sequence : |
1 MGSSHHHHHH SSGLVPRGSH MLEKIYDLHK KRSCNLDEQQ ELVERDDEKQ LMNVEPIHAD 61 ILLETYKRKI ADEGRLFLAE FQSIPRVFSK FPIKEARKPF NQNKNRYVDI LPYDYNRVEL 121 SEINGDAGSN YINASYIDGF KEPRKYIAAQ GPRDETVDDF WRMIWEQKAT VIVMVTRCEE 181 GNRNKCAEYW PSMEEGTRAF GDVVVKINQH KRCPDYIIQK LNIVNKKEKA TGREVTHIQF 241 TSWPDHGVPE DPHLLLKLRR RVNAFSNFFS GPIVVHCSAG VGRTGTYIGI DAMLEGLEAE 301 NKVDVYGYVV KLRRQRCLMV QVEAQYILIH QALVEYNQFG ETEVNLSELH PYLHNMKKRD 361 PPSEPSPLEA EFQRLPSYRS WRTQHIGNQE ENKSKNRNSN VIPYDYNRVP LKHELEMSKE 421 SEHDSDESSD DDSDSEEPSK YINASFIMSY WKPEVMIAAQ GPLKETIGDF WQMIFQRKVK 481 VIVMLTELKH GDQEICAQYW GEGKQTYGDI EVDLKDTDKS STYTLRVFEL RHSKRKDSRT 541 VYQYQYTNWS VEQLPAEPKE LISMIQVVKQ KLPQKNSSEG NKHHKSTPLL IHCRDGSQQT 601 GIFCALLNLL ESAETEEVVD IFQVVKALRK ARPGMVSTFE QYQFLYDVIA STYPAQNGQV 661 KKNNHQEDKI EFDNEVDKVK QDANCVNPLG APEKLPEAKE QAEGSEPTSG TEGPEHSVNG 721 PASPALNQGS |
| Purity : |
Purity 39.2% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
