Active Recombinant Human RAD51, His-tagged
Cat.No. : | RAD51-128H |
Product Overview : | Active Recombinant Human RAD51, fused with His tag, was expressed in E.coli. |
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Source : | E.coli |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, 0.01% SKL, pH 7.4, 5% Trehalose, 5% Mannitol. |
Bio-activity : | The EC50 of the RAD51 is 16.3607ug/ml in the presence of 1ug of RAD51 antibody. |
Molecular Mass : | The protein has a calculated MW of 41 kDa. |
AA Sequence : | MGSSHHHHHHSSGLVPRGSHMASMT GGQQMGRGSEFAMQMQLEANADTSV EEESFGPQPISRLEQCGINANDVKK LEEAGFHTVEAVAYAPKKELINIKG ISEAKADKILAEAAKLVPMGFTTAT EFHQRRSEIIQITTGSKELDKLLQG GIETGSITEMFGEFRTGKTQICHTL AVTCQLPIDRGGGEGKAMYIDTEGT FRPERLLAVAERYGLSGSDVLDNVA YARAFNTDHQTQLLYQASAMMVESR YALLIVDSATALYRTDYSGRGELSA RQMHLARFLRMLLRLADEFGVAVVI TNQVVAQVDGAAMFAADPKKPIGGN IIAHASTTRLYLRKGRGETRICKIY DSPCLPEAEAMFAINADGVGDAKD |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 90% as determined by SDS-PAGE. |
Storage : | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | RAD51 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) [ Homo sapiens ] |
Official Symbol : | RAD51 |
Synonyms : | RAD51; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RECA; BRCC5; HRAD51; RAD51A; HsRad51; HsT16930; RAD51 homolog protein; RecA-like protein; RAD51D; recombination protein A; RecA, E. coli, homolog of DNA repair protein RAD51 homolog 1; BRCA1/BRCA2-containing complex, subunit 5; RAD51L3; hRAD51; RAD51 (S. cerevisiae) homolog (E coli RecA homolog) |
Gene ID : | 5888 |
mRNA Refseq : | NM_002875 |
Protein Refseq : | NP_002866 |
MIM : | 179617 |
UniProt ID : | Q06609 |
Products Types
◆ Lysates | ||
RAD51-2556HCL | Recombinant Human RAD51 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionIn the HR pathway, RAD51 forms nucleoprotein filaments on single-stranded DNA, allowing it to search for and invade a homologous DNA sequence, facilitating accurate DNA repair.
Yes, RAD51 interacts with BRCA proteins, especially BRCA2. This interaction is crucial for the proper function of RAD51 in the HR pathway.
While mutations in RAD51 are rare, dysregulation of its function can contribute to genomic instability. Some mutations in RAD51 have been linked to increased cancer susceptibility.
RAD51 plays a central role in the DNA repair mechanism, specifically in homologous recombination (HR), where it facilitates the search for homology and strand pairing.
RAD51 is recruited to sites of DNA damage through interactions with various mediator proteins, including BRCA2, which helps load RAD51 onto single-stranded DNA regions.
Yes, RAD51's activity can be targeted therapeutically. Inhibiting RAD51 can sensitize cancer cells to DNA-damaging agents, making it a potential target in combination cancer therapies, especially for tumors with defective DNA repair pathways.
RAD51 has several paralogs, including RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3. While they all play roles in HR, each has distinct functions and interactions, with some acting as mediators or stabilizers of RAD51's nucleoprotein filament.
Customer Reviews (4)
Write a reviewRAD51-134H, Rad51 works well.
User-friendly protocol included.
Affordable price, great value.
Product met all specifications.
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