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Active Recombinant Human Retinoblastoma 1

Cat.No. : RB1-1192H
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Cat. No. : RB1-1192H
Description : PRB, the product of retinoblastoma susceptibility gene RB-1, is one of the best-studied tumor suppressor gene products. At least two other pRB-related proteins, p107 and p130, have been identified and characterized. Mutations in RB-1 gene are often associated with the occurrence of various tumors. The activity of pRB is regulated through phosphorylation in a cell cycle-dependent manner. The hyperphosphorylated RB protein usually associates with the cell nucleus and binds transcription factors of E2F family. pRB represses transcription of its target genes, such as cdc2, cyclin A, and oncogene c-myc, c-fos through the binding with E2F factors and thereby regulating cell proliferation. The wild type pRB (residue1-928) is expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography. Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase, and RNase activity.
Source : Sf9 insect cells.
Usage : For in vitro use only.
Application : Recombinant pRB protein can be used for (1) in vitro function studies including transcription and DNA binding assays, (2) protein-protein interaction assay and (3) cell growth and proliferation assays.
Form : Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
Activity : 1 ng is sufficient for a gel mobility shift assay in a 20μl reaction, 50 ng are sufficient for reconstituted transcription assays and 100 ng are sufficient for a protein-protein interaction assay.
Purity : > 95% by SDS-PAGE.
Storage : Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.
Gene Name : RB1 retinoblastoma 1 [ Homo sapiens ]
Synonyms : RB1; retinoblastoma 1; RB; pRb; OSRC; pp110; p105-Rb; RB1; osteosarcoma; OTTHUMP00000018397; Rb; retinoblastoma suspectibility protein; retinoblastoma-associated protein
Gene ID : 5925
mRNA Refseq : NM_000321
Protein Refseq : NP_000312
MIM : 180200
UniProt ID : P06400
Chromosome Location : 13q14.2
Pathway : Bladder cancer; Cell cycle; Chronic myeloid leukemia; Glioma; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Small cell lung cancer; Cell Cycle, Mitotic; DNA Replication
Function : androgen receptor binding; kinase binding; phosphoprotein binding; transcription coactivator activity; transcription factor activity; transcription factor binding; transcription repressor activity; ubiquitin protein ligase binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the impact of RB1 mutations on the development of retinoblastoma and other cancers? 02/14/2022

Mutations in RB1 lead to retinoblastoma and increase the risk of other cancers, disrupting normal cell cycle control.

How does RB1 interact with transcription factors and affect gene expression? 10/30/2021

RB1 interacts with transcription factors to regulate gene expression, impacting various cellular processes.

What role does RB1 play in DNA damage response and repair mechanisms? 02/25/2021

RB1 plays a crucial role in the DNA damage response, facilitating repair and maintaining genomic stability.

How does RB1 regulate the cell cycle, particularly the transition from G1 to S phase? 04/09/2020

RB1 controls the G1 to S phase transition in the cell cycle, preventing unregulated cell division.

How do alterations in RB1 expression or function influence stem cell differentiation and tissue regeneration? 10/25/2019

Changes in RB1 function affect stem cell differentiation and tissue regeneration, influencing tissue maintenance and repair.

What are the mechanisms by which RB1 modulates cellular senescence and apoptosis? 09/03/2019

RB1 influences cellular senescence and apoptosis, playing a role in aging and cell turnover.

How does RB1 contribute to tumor suppression and the prevention of uncontrolled cell proliferation? 02/17/2019

As a tumor suppressor, RB1 prevents excessive cell proliferation and contributes to cancer prevention.

Customer Reviews (3)

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Reviews
07/15/2022

    Efficient protein purification, delivers consistent yield and purity.

    01/19/2021

      Expertise in protein engineering, a trusted resource for research.

      10/09/2019

        Reliable protein crystallization services, exceptional results achieved.

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