Active Recombinant Human Retinoblastoma 1
Cat.No. : | RB1-1192H |
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Cat. No. : | RB1-1192H |
Description : | PRB, the product of retinoblastoma susceptibility gene RB-1, is one of the best-studied tumor suppressor gene products. At least two other pRB-related proteins, p107 and p130, have been identified and characterized. Mutations in RB-1 gene are often associated with the occurrence of various tumors. The activity of pRB is regulated through phosphorylation in a cell cycle-dependent manner. The hyperphosphorylated RB protein usually associates with the cell nucleus and binds transcription factors of E2F family. pRB represses transcription of its target genes, such as cdc2, cyclin A, and oncogene c-myc, c-fos through the binding with E2F factors and thereby regulating cell proliferation. The wild type pRB (residue1-928) is expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography. Purified protein is greater than 95% homogeneous and contains no detectable proteases, DNase, and RNase activity. |
Source : | Sf9 insect cells. |
Usage : | For in vitro use only. |
Application : | Recombinant pRB protein can be used for (1) in vitro function studies including transcription and DNA binding assays, (2) protein-protein interaction assay and (3) cell growth and proliferation assays. |
Form : | Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol. |
Activity : | 1 ng is sufficient for a gel mobility shift assay in a 20μl reaction, 50 ng are sufficient for reconstituted transcription assays and 100 ng are sufficient for a protein-protein interaction assay. |
Purity : | > 95% by SDS-PAGE. |
Storage : | Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles. |
Gene Name : | RB1 retinoblastoma 1 [ Homo sapiens ] |
Synonyms : | RB1; retinoblastoma 1; RB; pRb; OSRC; pp110; p105-Rb; RB1; osteosarcoma; OTTHUMP00000018397; Rb; retinoblastoma suspectibility protein; retinoblastoma-associated protein |
Gene ID : | 5925 |
mRNA Refseq : | NM_000321 |
Protein Refseq : | NP_000312 |
MIM : | 180200 |
UniProt ID : | P06400 |
Chromosome Location : | 13q14.2 |
Pathway : | Bladder cancer; Cell cycle; Chronic myeloid leukemia; Glioma; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Small cell lung cancer; Cell Cycle, Mitotic; DNA Replication |
Function : | androgen receptor binding; kinase binding; phosphoprotein binding; transcription coactivator activity; transcription factor activity; transcription factor binding; transcription repressor activity; ubiquitin protein ligase binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMutations in RB1 lead to retinoblastoma and increase the risk of other cancers, disrupting normal cell cycle control.
RB1 interacts with transcription factors to regulate gene expression, impacting various cellular processes.
RB1 plays a crucial role in the DNA damage response, facilitating repair and maintaining genomic stability.
RB1 controls the G1 to S phase transition in the cell cycle, preventing unregulated cell division.
Changes in RB1 function affect stem cell differentiation and tissue regeneration, influencing tissue maintenance and repair.
RB1 influences cellular senescence and apoptosis, playing a role in aging and cell turnover.
As a tumor suppressor, RB1 prevents excessive cell proliferation and contributes to cancer prevention.
Customer Reviews (3)
Write a reviewEfficient protein purification, delivers consistent yield and purity.
Expertise in protein engineering, a trusted resource for research.
Reliable protein crystallization services, exceptional results achieved.
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