Active Recombinant Human Retinoblastoma 1, His-tagged

• Cat.No.: RB1-2509H
• Product Overview: Recombinant human His-tagged pRB (residues 1-928) is expressed in abaculovirussystem and purified by affinity and FPLC chromatography.105 kDa.

Specification

• Cat. No.: RB1-2509H
• Description: pRB, the product of retinoblastoma susceptibility gene RB-1, is one of the best-studied tumor suppressor gene products. At least two other pRB-related proteins, p107 and p130, have been identified and characterized. Mutations in RB-1 gene are often associated with the occurrence of various tumors. The activity of pRB is regulated through phosphorylation in a cell cycle-dependent manner. The hyperphosphorylated RB protein usually associates with the cell nucleus and binds transcription factors of the E2F family. pRB represses transcription of its target genes, such as cdc2, cyclin A, c-Fos and oncogene c-Myc, through the binding with E2F factors and thereby regulating cell proliferation.
• Source: baculovirus.
• Applications: Recombinant pRB protein can be used for: 1) in vitro function studies including transcription and DNA binding assays; 2) protein-protein interaction assay; and 3) cell growth and proliferation assays.
• Activity: 1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
• Quality Control: The purified protein is greater than 95% homogeneous based on SDS-PAGE gel analysis.
• Reagents Supplied: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT.
• Storage Conditions: Store at -80°C.
• Pathways: Bladder cancer; Cell cycle; Chronic myeloid leukemia; Glioma; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Small cell lung cancer; Cell Cycle, Mitotic; DNA Replication

Gene Information

• Gene Name: RB1 retinoblastoma 1 [ Homo sapiens ]
• Synonyms: RB1; retinoblastoma 1; RB; pRb; OSRC; pp110; p105-Rb; RB1; osteosarcoma; OTTHUMP00000018397; Rb; retinoblastoma suspectibility protein; retinoblastoma-associated protein
• Gene ID: 5925
• mRNA Refseq: NM_000321
• Protein Refseq: NP_000312
• MIM: 180200
• UniProt ID: P06400
• Chromosome Location: 13q14.2
• Function: androgen receptor binding; kinase binding; phosphoprotein binding; transcription coactivator activity; transcription factor activity; transcription factor binding; transcription repressor activity; ubiquitin protein ligase binding

Reviews

07/15/2022

Efficient protein purification, delivers consistent yield and purity.

01/19/2021

Expertise in protein engineering, a trusted resource for research.

10/09/2019

Reliable protein crystallization services, exceptional results achieved.

Q&As

What is the impact of RB1 mutations on the development of retinoblastoma and other cancers?

Mutations in RB1 lead to retinoblastoma and increase the risk of other cancers, disrupting normal cell cycle control.

How does RB1 interact with transcription factors and affect gene expression?

RB1 interacts with transcription factors to regulate gene expression, impacting various cellular processes.

What role does RB1 play in DNA damage response and repair mechanisms?

RB1 plays a crucial role in the DNA damage response, facilitating repair and maintaining genomic stability.

How does RB1 regulate the cell cycle, particularly the transition from G1 to S phase?

RB1 controls the G1 to S phase transition in the cell cycle, preventing unregulated cell division.

How do alterations in RB1 expression or function influence stem cell differentiation and tissue regeneration?

Changes in RB1 function affect stem cell differentiation and tissue regeneration, influencing tissue maintenance and repair.

What are the mechanisms by which RB1 modulates cellular senescence and apoptosis?

RB1 influences cellular senescence and apoptosis, playing a role in aging and cell turnover.

How does RB1 contribute to tumor suppression and the prevention of uncontrolled cell proliferation?

As a tumor suppressor, RB1 prevents excessive cell proliferation and contributes to cancer prevention.