Active Recombinant Human RHOT1 protein, His-tagged

• Cat.No.: RHOT1-261H
• Product Overview: Recombinant Human RHOT1(Ile186 - Thr591) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa. Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules. A number of publications have identifed Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential component of the cellular machinery that participates in the removal of damaged mitochondria. This protein is useful as an in vitro substrate for activated recombinant Parkin enzyme.
• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (X μM) in 50 mM HEPES, 200 mM NaCl, 2 mM TCEP
• Bio-activity: Reaction Conditions will be optimized for each specific application. In a 50 μl reaction containing 1 μM activated Parkin E3 ligase, 1 μM His6-Miro1 is fully converted to Ubiquitinated forms in 60 minutes.
• Molecular Mass: Predicted Molecular Mass: 50 kDa
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: RHOT1 ras homolog family member T1 [ Homo sapiens ]
• Official Symbol: RHOT1
• Synonyms: RHOT1; ras homolog family member T1; ARHT1, ras homolog gene family, member T1; mitochondrial Rho GTPase 1; FLJ11040; MIRO 1; mitochondrial Rho (MIRO) GTPase 1; hMiro-1; mitochondrial Rho 1; ras homolog gene family, member T1; rac-GTP binding protein-like protein; rac-GTP-binding protein-like protein; ARHT1; MIRO1; MIRO-1; FLJ12633
• Gene ID: 55288
• mRNA Refseq: NM_001033566
• Protein Refseq: NP_001028738
• MIM: 613888
• UniProt ID: Q8IXI2