Active Recombinant Human RSPO1 Protein (Ser21-Ala263), N-His-SUMO tagged, Animal-free, Carrier-free

• Cat.No.: RSPO1-132H
• Product Overview: Recombinant Human RSPO1 Protein (Ser21-Ala263) with N-His-SUMO tag was expressed in HEK293 and Animal-free as well as Carrier-free.

Specification

• Description: R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/ 6), subsequent clearing negative regulators ZNRF3 /RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.
• Source: HEK293 cell
• Species: Human
• Tag: N-His-SUMO
• Form: Liquid
• Bio-activity: R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED50 for this effect is 1-3 μg/mL.
• Molecular Mass: The protein has a calculated MW of 46.8 kDa.
  The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ser21-Ala263
• Endotoxin: < 1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -80 centigrade for long-term storage under sterile conditions.
  Avoid repeated free-thaw cycles.
• Storage Buffer: Phosphate buffered saline, pH 7.4.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens (human) ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1
• Gene ID: 284654
• mRNA Refseq: NM_001038633
• Protein Refseq: NP_001033722
• MIM: 609595
• UniProt ID: Q2MKA7