Active Recombinant Human SELE Protein, His-tagged

• Cat.No.: SELE-05H
• Product Overview: Recombinant human E selectin (22-556aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.
• Source: Insects cells
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human E-Seletin/CD62E coated plates 2 μg/mL. This effect is more to 40%.
• Molecular Mass: 59.4kDa(541aa)
• AA Sequence: WSYNTSTEAMTYDEASAYCQQRYTH LVAIQNKEEIEYLNSILSYSPSYYW IGIRKVNNVWVWVGTQKPLTEEAKN WAPGEPNNRQKDEDCVEIYIKREKD VGMWNDERCSKKKLALCYTAACTNT SCSGHGECVETINNYTCKCDPGFSG LKCEQIVNCTALESPEHGSLVCSHP LGNFSYNSSCSISCDRGYLPSSMET MQCMSSGEWSAPIPACNVVECDAVT NPANGFVECFQNPGSFPWNTTCTFD CEEGFELMGAQSLQCTSSGNWDNEK PTCKAVTCRAVRQPQNGSVRCSHSP AGEFTFKSSCNFTCEEGFMLQGPAQ VECTTQGQWTQQIPVCEAFQCTALS NPERGYMNCLPSASGSFRYGSSCEF SCEQGFVLKGSKRLQCGPTGEWDNE KPTCEAVRCDAVHQPPKGLVRCAHS PIGEFTYKSSCAFSCEEGFELHGST QLECTSQGQWTEEVPSCQVVKCSSL AVPGKINMSCSGEPVFGTVCKFACP EGWTLNGSAARTCGATGHWSGLLPT CEAPTESNIP
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: SELE selectin E [ Homo sapiens (human) ]
• Official Symbol: SELE
• Synonyms: SELE; selectin E; ELAM; ESEL; CD62E; ELAM1; LECAM2; E-selectin; CD62 antigen-like family member E; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2
• Gene ID: 6401
• mRNA Refseq: NM_000450
• Protein Refseq: NP_000441
• MIM: 131210
• UniProt ID: P16581