| Description : |
Neuroserpin is an inhibitory serpin that is expressed predominantly in central nervous system. Although the physiological target of neuroserpin is still unclear, cumulative evidence suggest that it plays an important role in controlling proteolytic degradation of extracellular matrix (ECM) during synaptogenesis and the subsequent development of neuronal plasticity. In the adult brain, neuroserpin is secreted from the growth cones of neurons in areas where synaptic changes are associated with learning and memory, i.e. cerebral cortex, hippocampus, and amygdala. The neuroprotective role of neuroserpin has been demonstrated in transgenic mice lacking neuroserpin expression. The deficiency of neuroserpin in these mice was associated with motor neuron disease characterized by axonal degradation. In humans, defects in neuroserpin, caused by point mutations in the neuroserpin gene, underlie a hereditary disorder called the familial encephalopathy with neuroserpin inclusion bodies (FENIB). |
| Source : |
CHO |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 2 μg/mL, measured by the dose-dependent stimulation of the proliferation of rat C6 cells, corresponding to a specific activity of > 500 units/mg. |
| Molecular Mass : |
40-45 kDa, observed by non-reducing SDS-PAGE. |
| AA Sequence : |
TGATFPEEAIADLSVNMYNRLRATG EDENILFSPLSIALAMGMMELGAQG STQKEIRHSMGYDSLKNGEEFSFLK EFSNMVTAKESQYVMKIANSLFVQN GFHVNEEFLQMMKKYFNAAVNHVDF SQNVAVANYINKWVENNTNNLVKDL VSPRDFDAATYLALINAVYFKGNWK SQFRPENTRTFSFTKDDESEVQIPM MYQQGEFYYGEFSDGSNEAGGIYQV LEIPYEGDEISMMLVLSRQEVPLAT LEPLVKAQLVEEWANSVKKQKVEVY LPRFTVEQEIDLKDVLKALGITEIF IKDANLTGLSDNKEIFLSKAIHKSF LEVNEEGSEAAAVSGMIAISRMAVL YPQVIVDHPFFFLIRNRRTGTILFM GRVMHPETMNTSGHDFEEL |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant Human Neuroserpin remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rh_Neuroserpin should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
