Active Recombinant Human SGK1(S422D)(60-end) Mutation Protein, N-His-tagged

• Cat.No.: SGK1-222H
• Product Overview: N-terminal 6His-tagged, recombinant, human SGK1, amino acids 60–end, containing an S422D mutation. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose. Activated with PDK1 and repurified using heparin-Sepharose.

Specification

• Description: The serum/glucocorticoid regulated kinases (SGK1,2,3) are serine/threonine kinases that belong to the AGC (protein kinase A; protein kinase G and protein kinase C) subfamily that includes cAMP and cGMP dependent kinases, PKB and PKC. SGK1 (SGK) is predominately expressed in kidney, liver and pancreas. The catalytic domain of serum/glucocorticoid regulated protein kinase is most similar to PKB and activated via phosphorylation mechanism when cells are stimulated with insulin, IGF1 or serum, or exposed to oxidative stress. GenBank AF153609. The protein is S422D with respect to this accession number.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 60-end
• Form: 1.587mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 5120U/mg, where one unit of SGK1 (S422D), active activity is defined as 1nmol phosphate incorporated into 30μM modified crosstide (GRPRTSSFAEGKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 45.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGISQ PQEPELMNAN PSPPPSPSQQ INLGPSSNPH 61 AKPSDFHFLK VIGKGSFGKV LLARHKAEEV FYAVKVLQKK AILKKKEEKH IMSERNVLLK 121 NVKHPFLVGL HFSFQTADKL YFVLDYINGG ELFYHLQRER CFLEPRARFY AAEIASALGY 181 LHSLNIVYRD LKPENILLDS QGHIVLTDFG LCKENIEHNS TTSTFCGTPE YLAPEVLHKQ 241 PYDRTVDWWC LGAVLYEMLY GLPPFYSRNT AEMYDNILNK PLQLKPNITN SARHLLEGLL 301 QKDRTKRLGA KDDFMEIKSH VFFSLINWDD LINKKITPPF NPNVSGPNDL RHFDPEFTEE 361 PVPNSIGKSP DSVLVTASVK EAAEAFLGFD YAPPTDSFL
• Purity: Purity 98.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SGK1 serum/glucocorticoid regulated kinase 1 [ Homo sapiens (human) ]
• Official Symbol: SGK1
• Synonyms: SGK1; serum/glucocorticoid regulated kinase 1; SGK
• Gene ID: 6446
• mRNA Refseq: NM_005627.4
• Protein Refseq: NP_005618.2
• MIM: 602958
• UniProt ID: O00141