Active Recombinant Human SIGLEC15 protein, His-Avi-tagged, Biotinylated

• Cat.No.: SIGLEC15-0675H
• Product Overview: Biotinylated Recombinant Human SIGLEC15 protein(Phe20-Thr263), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in 25 mM MES,150 mM NaCl, pH5.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse Human chimeric MAb (5G12, Human IgG1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-15 Protein, His,Avitag with a linear range of 0.2-3 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 30.1 kDa. The protein migrates as 33-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe20-Thr263
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.