Active Recombinant Human SIK1(1-281) Protein, N-His-tagged

• Cat.No.: SIK1-227H
• Product Overview: N-terminal 6Histagged, recombinant, human SIK, amino acids 1–281, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: SIK plays a transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. This protein has a potential role in G2/M cell cycle regulation, and inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3 (By similarity).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-281 aa
• Form: 0.918mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 451U/mg, where one unit of SIK, active activity is defined as 1nmol phosphate incorporated into 100μM (AMARAASAAALARRR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 36.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MVIMSEFSAD PAGQGQGQQK PLRVGFYDIE 61 RTLGKGNFAV VKLARHRVTK TQVAIKIIDK TRLDSSNLEK IYREVQLMKL LNHPHIIKLY 121 QVMETKDMLY IVTEFAKNGE MFDYLTSNGH LSENEARKKF WQILSAVEYC HDHHIVHRDL 181 KTENLLLDGN MDIKLADFGF GNFYKSGEPL STWCGSPPYA APEVFEGKEY EGPQLDIWSL 241 GVVLYVLVCG SLPFDGPNLP TLRQRVLEGR FRIPFFMSQD CESLIRRMLV VDPARRITIA 301 QIRQHRWMRA E
• Purity: Purity 52% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SIK1 salt inducible kinase 1 [ Homo sapiens (human) ]
• Official Symbol: SIK1
• Synonyms: SIK1; salt inducible kinase 1; MSK; SIK; DEE30; SIK-1; SIK1B; SNF1LK
• Gene ID: 150094
• mRNA Refseq: NM_173354.5
• Protein Refseq: NP_775490.2
• MIM: 605705
• UniProt ID: P57059

Reviews

08/14/2021

Our lab swears by this product! It's a versatile and reliable tool for our experiments.

06/05/2019

The quality control is evident in the data precision we achieve.

12/09/2018

As a researcher, I can't live without this product. Makes my experiments more accurate and efficient.

Q&As

What is the significance of SIK1 dysregulation in the context of cancer, and how does it impact cell proliferation and survival?

Dysregulation of SIK1 has been implicated in cancer. It can influence cell proliferation and survival by affecting the activity of transcription factors involved in cell cycle control and apoptosis.

How does SIK1 contribute to the regulation of adipose tissue metabolism, and what implications does this have for metabolic disorders?

SIK1 plays a role in adipose tissue metabolism by modulating the activity of transcription factors involved in lipogenesis and lipolysis. Dysregulation may contribute to metabolic disorders like obesity and insulin resistance.

What is the primary function of the SIK1 protein in cellular processes?

SIK1, or Salt-Inducible Kinase 1, is involved in the regulation of cellular responses to various stimuli, including stress, metabolism, and inflammation.

How does SIK1 participate in cellular energy homeostasis, and what are its downstream targets in metabolic pathways?

SIK1 regulates energy homeostasis by phosphorylating and modulating the activity of key metabolic regulators, such as cAMP response element-binding protein (CREB) and class IIa histone deacetylases.

How does SIK1 contribute to cellular responses to environmental stress, and what are the molecular mechanisms involved in stress signaling pathways?

SIK1 is activated in response to environmental stressors, such as osmotic stress. It regulates stress signaling pathways through the phosphorylation of downstream effectors, modulating cellular stress responses.