Active Recombinant Human SIK3(1-307) Protein, N-GST,C-His-tagged

• Cat.No.: SIK3-229H
• Product Overview: N-terminal GST and C-terminal 6His-tagged, recombinant, human SIK3 amino acids 1- 307 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: SIK (Myocardial Snf1-Like Kinase or Msk) was initially identified as a Ser/Thr kinase with homology to yeast Snf1 that is involved in mouse cardiogenesis. The human gene maps to a chromosomal region associated with congenital heart defects in Down's syndrome. Snf1 is a histone kinase (H3, Ser10) that may be involved in transcriptional regulation; a role for SIK in histone modification has not yet been firmly established. SIK3 IS involved in positive regulation of TORC1 signaling, positive regulation of TORC2 signaling, and protein phosphorylation.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: 1-307 aa
• Form: 0.37 mg/ml of enzyme in 25 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.05 mM EGTA, 50% (v/v) glycerol, 75 mM imidazole, 0.5 mM benzamidine, 0.1 mM PMSF, 0.05% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 444 U/mg, where one unit of SIK3 activity is defined as 1 nmol phosphate incorporated into 100 μM CHKtide (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 63 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFM PARIGYYEID 241 RTIGKGNFAV VKRATHLVTK AKVAIKIIDK TQLDEENLKK IFREVQIMKM LCHPHIIRLY 301 QVMETERMIY LVTEYASGGE IFDHLVAHGR MAEKEARRKF KQIVTAVYFC HCRNIVHRDL 361 KAENLLLDAN LNIKIADFGF SNLFTPGQLL KTWCGSPPYA APELFEGKEY DGPKVDIWSL 421 GVVLYVLVCG ALPFDGSTLQ NLRARVLSGK FRIPFFMSTE CEHLIRHMLV LDPNKRLSME 481 QICKHKWMKL GDADPNFDRL IAECQQLKEE RQVDPLNEDV LLAMEDMGLD KEQTLQGPGP 541 GHHHHHH
• Purity: Purity 76% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SIK3 SIK family kinase 3 [ Homo sapiens (human) ]
• Official Symbol: SIK3
• Synonyms: SIK3; SIK family kinase 3; L19; QSK; SEMDK; SIK-3
• Gene ID: 23387
• mRNA Refseq: NM_025164.6
• Protein Refseq: NP_079440.3
• MIM: 614776
• UniProt ID: Q9Y2K2