Active Recombinant Human SIRT6 Protein, GST-tagged

• Cat.No.: SIRT6-28517H
• Product Overview: Human Sirtuin 6 (GenBank Accession No. NM_016539), full length with N-terminal GST-tag was expressed in an E. coli expression system.

Specification

• Description: This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E.coli
• Species: Human
• Tag: GST
• Form: Aqueous buffer solution
• Bio-activity: 1.5 pmol/min/µg
  Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS SIRT6 substrate 2, 500 µM NAD+ and Sirtuin 6 (18 – 2000 ng). Incubation condition: 60 min at 37ºC followed by developing for 15 min at room temperature.
• Molecular Mass: 65 kDa
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: At least 6 months at –80 centigrade. Avoid freeze/thaw cycles.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: SIRT6 sirtuin 6 [ Homo sapiens (human) ]
• Official Symbol: SIRT6
• Synonyms: SIRT6; sirtuin 6; SIR2L6; NAD-dependent protein deacetylase sirtuin-6; SIR2-like protein 6; regulatory protein SIR2 homolog 6; sir2-related protein type 6; sirtuin type 6; EC 2.4.2.31
• Gene ID: 51548
• mRNA Refseq: NM_016539
• Protein Refseq: NP_057623
• MIM: 606211
• UniProt ID: Q8N6T7