Active Recombinant Human SOD1

• Cat.No.: SOD1-1432H
• Product Overview: Recombinant Human Superoxide Dismutase produced in E. coli. is a stable dimer of two identical subunits, non-glycosylated, containing 308 amino acid residues, two pairs of disulfide bonds and having a combined molecular mass of 31.6kDa.

Specification

• Description: Cu/Zn Human Superoxide Dismutase is a stable dimer of identical subunits with a combined molecular mass of 31.6kD. This enzyme dismutes the superoxide radical to molecular oxygen. This enzyme has been expressed in E. coli and purified using sequential chromatography steps.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2μm filtered solution in 50mM Phosphate buffer, pH7.4.
• Bio-activity: ≥7000U/mg
• Endotoxin: ≤1EU/μg, determined by the LAL method.
• Purity: ≥95%, as determined by reduced SDS-PAGE Dimer ≥90%, as determined by SEC-HPLC.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuSOD in sterile ddH2O.

Gene Information

• Gene Name: SOD1 superoxide dismutase 1, soluble [ Homo sapiens ]
• Official Symbol: SOD1
• Synonyms: ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer; superoxide dismutase [Cu-Zn]; Cu/Zn superoxide dismutase; SOD, soluble;epididymis secretory protein Li 44; indophenoloxidase A; superoxide dismutase, cystolic
• Gene ID: 6647
• mRNA Refseq: NM_000454
• Protein Refseq: NP_000445
• MIM: 147450
• UniProt ID: P00441
• Chromosome Location: 21q22.11
• Pathway: AGE/RAGE pathway, organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Detoxification of Reactive Oxygen Species, organism-specific biosystem
• Function: Rac GTPase binding; chaperone binding; copper ion binding

Reviews

11/15/2022

Worked perfectly, no problems.

10/09/2020

Quick delivery, fantastic product.

10/02/2020

Reliable results every time.

Q&As

Are there potential therapeutic strategies targeting SOD1 for disease treatment?

Therapeutic strategies may involve modulating SOD1 activity or expression to mitigate oxidative stress-related damage.

What is the primary biological function of SOD1 in cells?

SOD1 acts as an antioxidant, converting superoxide radicals to hydrogen peroxide and oxygen.

What are the genetic implications of mutations in the SOD1 gene?

Mutations in SOD1 can result in loss or gain of function, contributing to disease development.

How is SOD1 implicated in neurodegenerative diseases?

SOD1 mutations are implicated in Amyotrophic Lateral Sclerosis (ALS), leading to neurodegeneration.

In which cellular compartments is SOD1 primarily located?

SOD1 is primarily located in the cytoplasm and mitochondrial intermembrane space.

What role does SOD1 play in maintaining cellular redox balance?

It helps maintain cellular redox balance, protecting cells from oxidative damage.

How does SOD1 contribute to the cellular antioxidant defense system?

It plays a crucial role in cellular defense against oxidative stress.