Active Recombinant Human TEK(Y897S)(771-end) Mutation Protein, N-His-tagged

• Cat.No.: TEK-245H
• Product Overview: N-terminal 6His-tagged recombinant human Tie2 residues 771–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose. The Y897S substitution is a gain-of-function mutation associated with hereditary venous malformations.

Specification

• Description: This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 771-end
• Form: 2.9mg/ml of enzyme in 50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: As provided, this lot demonstrated 123U/mg, where one unit of Tie2 (Y897S), active activity is defined as 1nmol phosphate incorporated into 400μM (EFPIYDFLPAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 42 kDa
• AA Sequence: 1 MHHHHHHEFQ LKRANVQRRM AQAFQNVREE PAVQFNSGTL ALNRKVKNNP DPTIYPVLDW 61 NDIKFQDVIG EGNFGQVLKA RIKKDGLRMD AAIKRMKEYA SKDDHRDFAG ELEVLCKLGH 121 HPNIINLLGA CEHRGSLYLA IEYAPHGNLL DFLRKSRVLE TDPAFAIANS TASTLSSHHL 181 LHFAADVARG MDYLSQKQFI HRDLAARNIL VGENYVAKIA DFGLSRGQEV YVKKTMGRLP 241 VRWMAIESLN YSVYTTNSDV WSYGVLLWEI VSLGGTPYCG MTCAELYEKL PQGYRLEKPL 301 NCDDEVYDLM RQCWREKPYE RPSFAQILVS LNRMLEERKT YVNTTLYEKF TYAGIDCSAE 361 EAA
• Purity: Purity 93% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TEK TEK receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: TEK
• Synonyms: TEK; TEK receptor tyrosine kinase; TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B
• Gene ID: 7010
• mRNA Refseq: NM_000459.5
• Protein Refseq: NP_000450.3
• MIM: 600221
• UniProt ID: Q02763