Active Recombinant Human TGFB2 Protein (Ala303-Ser414), C-His tagged, Animal-free, Carrier-free

• Cat.No.: TGFB2-287H
• Product Overview: Recombinant Human TGFB2 Protein (Ala303-Ser414) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Transforming Growth Factors beta 2 (TGFβ-2) is a 12.85 kDa member of the epidermal Growth Factors with 113 amino acid residues. TGFβ-2 is expressed from throughout the body. TGFβ-2 is a regulator of cell proliferation, differentiation, apoptosis, cell plasticity and migration, etc. TGF-β-2 also associates with various kinds of diseases, such as cancer and tissue fibrosis.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant human TGF beta 2 is > 5 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 13.66 kDa.
  The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ala303-Ser414
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: TGFB2 transforming growth factor, beta 2 [ Homo sapiens (human) ]
• Official Symbol: TGFB2
• Synonyms: TGFB2; transforming growth factor, beta 2; transforming growth factor beta-2; G-TSF; cetermin; polyergin; BSC-1 cell growth inhibitor; glioblastoma-derived T-cell suppressor factor; TGF-beta2; MGC116892
• Gene ID: 7042
• mRNA Refseq: NM_001135599
• Protein Refseq: NP_001129071
• MIM: 190220
• UniProt ID: P61812