Active Recombinant Human TGFBR1(T204D)(200-end) Mutation Protein, N-GST-tagged

• Cat.No.: TGFBR1-242H
• Product Overview: N-terminal GST-tagged, recombinant, human TGFBR1 amino acids 200–end containing a T204D mutation, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. The T204D mutation has been demonstrated to enhance kinase activity in vitro.

Specification

• Description: Transforming growth factor, beta receptor type I (TGF-beta receptor I) forms a heteromeric complex with TGF-beta receptor type II when bound to TGF-beta. This complex transduces the TGF-beta signal from the cell surface to the cytoplasm. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta receptor I is found in all tissues, but is most abundant in placenta and least abundant in brain and heart tissue. TGF-beta receptor I is phosphorylated at basal levels in the absence of ligand binding and is multiply phosphorylated, mainly in the GS region. Defects in TGF-beta receptor I are the cause of Loeys-Dietz syndrome type 1A (LDS1A), also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS).
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 200–end
• Form: 1.04mg/ml of enzyme in 50mM Tris/HCl pH7.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 273U/mg, where one unit of TGFBR1 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 62 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGLRRQTIAR 241 DIVLQESIGK GRFGEVWRGK WRGEEVAVKI FSSREERSWF REAEIYQTVM LRHENILGFI 301 AADNKDNGTW TQLWLVSDYH EHGSLFDYLN RYTVTVEGMI KLALSTASGL AHLHMEIVGT 361 QGKPAIAHRD LKSKNILVKK NGTCCIADLG LAVRHDSATD TIDIAPNHRV GTKRYMAPEV 421 LDDSINMKHF ESFKRADIYA MGLVFWEIAR RCSIGGIHED YQLPYYDLVP SDPSVEEMRK 481 VVCEQKLRPN IPNRWQSCEA LRVMAKIMRE CWYANGAARL TALRIKKTLS QLSQQEGIKM
• Purity: Purity 98% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TGFBR1 transforming growth factor beta receptor 1 [ Homo sapiens (human) ]
• Official Symbol: TGFBR1
• Synonyms: TGFBR1; transforming growth factor beta receptor 1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; TBRI; ALK-5; LDS1A; LDS2A; TBR-i; TGFR-1; ACVRLK4; tbetaR-I
• Gene ID: 7046
• mRNA Refseq: NM_001130916.3
• Protein Refseq: NP_001124388.1
• MIM: 190181
• UniProt ID: P36897