Active Recombinant human TNFAIP3 protein, C-His and N-DDDDK tagged

• Cat.No.: TNFAIP3-25H
• Product Overview: Recombinant Active Human TNFAIP3 Protein (Full length, 2 to 790) with C-His and N-DDDDK tag was expressed in Baculovirus infected Sf9 cells.

Specification

• Description: This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene.
• Source: Baculovirus infected Sf9 cells
• Species: Human
• Tag: C-His and N-DDDDK
• Form: Liquid
• Bio-activity: Specific activity: 0.766 pmol/min/μg
  Assay Conditions: Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and various amount of TNFAIP3 in a volume of 50 μL. Reaction was incubated at 37 centigrade for 30 min and fluorescent signal was measured at excitation of 360 nm, and emission at 460 nm.
• Molecular Mass: 91 kDa including tags
• Protein Length: Full length, 2 to 790
• Purity: 79% SDS-PAGE
• Applications: SDS-PAGE; Functional Studies
• Storage: Store at -80 centigrade. Avoid freeze/thaw cycle. This product is an active protein and may elicit a biological response in vivo, handle with caution.
• Storage Buffer: pH: 8.00
  Constituents: 0.63% Tris HCl, 64% Sodium chloride, 0.017% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
• Shipping: Shipped on Dry Ice

Gene Information

• Gene Name: TNFAIP3 TNF alpha induced protein 3 [ Homo sapiens (human) ]
• Official Symbol: TNFAIP3
• Synonyms: TNFAIP3; TNF alpha induced protein 3; A20; AISBL; AIFBL1; OTUD7C; TNFA1P2; tumor necrosis factor alpha-induced protein 3; OTU domain-containing protein 7C; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha induced protein 3; zinc finger protein A20; EC 3.4.19.12
• Gene ID: 7128
• mRNA Refseq: NM_006290
• Protein Refseq: NP_006281
• MIM: 191163
• UniProt ID: P21580