Active Recombinant Human TNFSF10 Protein (114-281), Tag-Free

• Cat.No.: TNFSF10-03H
• Product Overview: Recombinant human TRAIL protein (114-281aa) was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: Tag Free
• Form: Liquid
• Bio-activity: Measured in a cytotoxicity assay using Jurkat human acute T cell leukemia cells. The ED50 range ≤1ng/ml.
• Molecular Mass: 19.6 kDa (169aa)
• Protein Length: 114-281
• AA Sequence: MVRERGPQRVAAHITGTRGRSNTLS SPNSKNEKALGRKINSWESSRSGHS FLSNLHLRNGELVIHEKGFYYIYSQ TYFRFQEEIKENTKNDKQMVQYIYK YTSYPDPILLMKSARNSCWSKDAEY GLYSIYQGGIFELKENDRIFVSVTN EHLIDMDHEASFFGAFLVG
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: 20 mM Tris-HCl buffer (pH 7.5) containing 300mM NaCl, 0.1mM DTT, 10% glycerol

Gene Information

• Gene Name: TNFSF10 TNF superfamily member 10 [ Homo sapiens (human) ]
• Official Symbol: TNFSF10
• Synonyms: TNFSF10; TNF superfamily member 10; TL2; APO2L; CD253; TANCR; TRAIL; Apo-2L; TNLG6A; tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand 6A; tumor necrosis factor superfamily member 10
• Gene ID: 8743
• mRNA Refseq: NM_003810
• Protein Refseq: NP_003801
• MIM: 603598
• UniProt ID: P50591