| Description : |
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
C-His/Avi |
| Bio-activity : |
Immobilized Human P53 R175H (HLA-A*02:01) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-P53 R175H (HLA-A*02:01) Antibody , hFc Tag with the EC50 of 9.7ng/mL determined by ELISA. See testing image for detail. |
| Molecular Mass : |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on the Tris-Bis PAGE result. |
| Protein Length : |
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and HMTEVVRHC peptide |
| Endotoxin : |
Less than 1 EU/μg by the LAL method. |
| Purity : |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Stability : |
Reconstituted protein stable at -80 centigrade for 12 months, 4 centigrade for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Storage Buffer : |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
| Reconstitution : |
Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water. |
| Shipping : |
Shipped at ambient temperature. |
