Active Recombinant Human TREM2 protein(Met1-Ser174), His&Avi-tagged, Biotinylated

• Cat.No.: TREM2-310H
• Product Overview: Biotinylated Recombinant Human TREM2 (NP_061838.1) (Met1-Ser174) was expressed in HEK293, fused with a C-terminal polyhistidine tag followed by an AVI tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His&Avi
• Protein length: Met1-Ser174
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized TREM-2 / TREM2 Antibody, Mouse MAb at 1 μg/mL (100 μL/well) can bind Human TREM-2 Protein (ECD, His & AVI Tag), Biotinylated, the EC50 is 16-45ng/mL.
• Molecular Mass: The recombinant human TREM2 consists of 182 amino acids and predicts a molecular mass of 20.69 kDa. It migRates as an approximately 34.12 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TREM2 triggering receptor expressed on myeloid cells 2 [ Homo sapiens ]
• Official Symbol: TREM2
• Synonyms: TREM2; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2a; TREM 2; Trem2a; Trem2b; Trem2c; triggering receptor expressed on monocytes 2; TREM-2
• Gene ID: 54209
• mRNA Refseq: NM_018965
• Protein Refseq: NP_061838
• MIM: 605086
• UniProt ID: Q9NZC2