Active Recombinant Human ULK1(1-314) Protein, N-His-tagged

• Cat.No.: ULK1-254H
• Product Overview: N-terminal 6His-tagged, recombinant, human ULK1 amino acids 1-314 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography followed by gel filtration.

Specification

• Description: Serine/threonine-protein kinase ULK1 involved in autophagy in response to starvation. It acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. It also acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-314 aa
• Form: 0.45mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2858U/mg, where one unit of ULK1 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 39 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MEPGRGGTET VGKFEFSRKD LIGHGAFAVV 61 FKGRHREKHD LEVAVKCINK KNLAKSQTLL GKEIKILKEL KHENIVALYD FQEMANSVYL 121 VMEYCNGGDL ADYLHAMRTL SEDTIRLFLQ QIAGAMRLLH SKGIIHRDLK PQNILLSNPA 181 GRRANPNSIR VKIADFGFAR YLQSNMMAAT LCGSPMYMAP EVIMSQHYDG KADLWSIGTI 241 VYQCLTGKAP FQASSPQDLR LFYEKNKTLV PTIPRETSAP LRQLLLALLQ RNHKDRMDFD 301 EFFHHPFLDA SPSVRKSPPV PVPSYPSSGS GSSSSSSSTS HLAS
• Purity: Purity 83% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ULK1 unc-51 like autophagy activating kinase 1 [ Homo sapiens (human) ]
• Official Symbol: ULK1
• Synonyms: ULK1; unc-51 like autophagy activating kinase 1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
• Gene ID: 8408
• mRNA Refseq: NM_003565.4
• Protein Refseq: NP_003556.2
• MIM: 603168
• UniProt ID: O75385