Active Recombinant Human ULK2(1-306) Protein, N-GST,C-His-tagged

• Cat.No.: ULK2-255H
• Product Overview: N-terminal GST and C-terminal 6His-tagged, recombinant, human, ULK2, amino acids 1–306. Expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: ULK2 (Unc-51 Like Autophagy Activating Kinase 2) is a Serine/threonine-protein kinase involved in autophagy in response to starvation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: 1-306 aa
• Form: 0.23mg/ml of enzyme in 50mM Tris/HCl pH7.2, 300mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution.
• Bio-activity: 439U/mg, where one unit of ULK2, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 62 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MEVVGDFEYS 241 KRDLVGHGAF AVVFRGRHRQ KTDWEVAIKS INKKNLSKSQ ILLGKEIKIL KELQHENIVA 301 LYDVQELPNS VFLVMEYCNG GDLADYLQAK GTLSEDTIRV FLHQIAAAMR ILHSKGIIHR 361 DLKPQNILLS YANRRKSSVS GIRIKIADFG FARYLHSNMM AATLCGSPMY MAPEVIMSQH 421 YDAKADLWSI GTVIYQCLVG KPPFQANSPQ DLRMFYEKNR SLMPSIPRET SPYLANLLLG 481 LLQRNQKDRM DFEAFFSHPF LEQGPVKKSC PVPVPMYSGS VSGSSCGSSP SCRFASHHHH 541 HH
• Purity: Purity 94% by SDS-PAGE and Coomassie staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ULK2 unc-51 like autophagy activating kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ULK2
• Synonyms: ULK2; unc-51 like autophagy activating kinase 2; ATG1B; Unc51.2
• Gene ID: 9706
• mRNA Refseq: NM_014683.4
• Protein Refseq: NP_055498.3
• MIM: 608650
• UniProt ID: Q8IYT8