Active Recombinant Human VCL protein, His-tagged

• Cat.No.: VCL-1871H
• Product Overview: Recombinant Human VCL protein(Pro 2 - Gln 1066), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human Vinculin, His Tag at 1 μg/mL (100 μL/well) can bind Vinculin Antibody with a linear range of 0.2-16 ng/mL.
• Molecular Mass: The protein has a calculated MW of 118.5 kDa. The protein migrates as 130-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Pro 2 - Gln 1066
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VCL vinculin [ Homo sapiens ]
• Official Symbol: VCL
• Synonyms: VCL; vinculin; metavinculin; MVCL; CMD1W; CMH15
• Gene ID: 7414
• mRNA Refseq: NM_003373
• Protein Refseq: NP_003364
• MIM: 193065
• UniProt ID: P18206