Active Recombinant Human VEGF165 protein, His-Avi-tagged, Biotinylated
Cat.No. : | VEGF165-562HB |
Product Overview : | Biotinylated Recombinant Human VEGF165 protein(Ala 27 - Arg 191), fused with His and Avi tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized Human VEGF R2, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, His,Avitag with a linear range of 2-39 ng/mL. Immobilized Anti-VEGF MAb, Human IgG1 (Avastin) at 5 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, His,Avitag with a linear range of 1-15.6 ng/mL. Immobilized Human VEGF R2, His Tag at 2 μg/mL (100 μL/well), can bind pre-mixed increasing concentrations of Bevacizumab and 0.05 μg/mL (100 μL/well) Biotinylated Human VEGF165, His,Avitag with a half maximal inhibitory concentration (IC50) of 0.74 μg/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 22.4 kDa (monomer). As a result of glycosylation, the protein migrates as 25-33 kDa (monomer) under reducing (R) condition, and 42-55 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE). |
Protein Length : | Ala 27 - Arg 191 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
◆ Recombinant Protein | ||
VEGF165-562H | Recombinant Human VEGF165 Protein, His/AVI-tagged | +Inquiry |
VEGF165-372H | Active Recombinant Human VEGF165 Protein | +Inquiry |
VEGF165-1026H | Recombinant Human VEGF165 protein | +Inquiry |
VEGF165-1001H | Active Recombinant Human VEGF165 protein, His-tagged | +Inquiry |
VEGF165-1026Z | Active Recombinant Zebrafish VEGF165 Protein | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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