Active Recombinant Human VRK2(1-336) Protein, N-His-tagged

• Cat.No.: VRK2-256H
• Product Overview: N-terminal 6His-tagged, recombinant, human VRK2 amino acids 1–336, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: VRK2 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This protein is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. Multiple alternatively spliced transcript variants have been found for this gene.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-336 aa
• Form: 0.60mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 52U/mg, where one unit of VRK2, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 43 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQPEVM PPKRNEKYKL PIPFPEGKVL 61 DDMEGNQWVL GKKIGSGGFG LIYLAFPTNK PEKDARHVVK VEYQENGPLF SELKFYQRVA 121 KKDCIKKWIE RKQLDYLGIP LFYGSGLTEF KGRSYRFMVM ERLGIDLQKI SGQNGTFKKS 181 TVLQLGIRML DVLEYIHENE YVHGDVKAAN LLLGYKNPDQ VYLADYGLSY RYCPNGNHKQ 241 YQENPRKGHN GTIEFTSLDA HKGVALSRRS DVEILGYCML RWLCGKLPWE QNLKDPVAVQ 301 TAKTNLLDEL PQSVLKWAPS GSSCCEIAQF LVCAHSLAYD EKPNYQALKK ILNPHGIPLG 361 PLDFSTKGQS INVHT
• Purity: Purity 94% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: VRK2 VRK serine/threonine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: VRK2
• Synonyms: VRK2; VRK serine/threonine kinase 2
• Gene ID: 7444
• mRNA Refseq: NM_006296.7
• Protein Refseq: NP_006287.2
• MIM: 602169
• UniProt ID: Q86Y07