Active Recombinant Influenza A H7N9 (A/Shanghai/2/2013) HA protein(Met1-Val524), His-tagged
Cat.No. : | HA-3322V |
Product Overview : | Recombinant Influenza A virus (A/Shanghai/2/2013(H7N9)) hemagglutinin (translated amino acid of EPI439502) (Met1-Val524) was expressed in Insect Cells with a C-terminal polyhistidine tag. |
- Specification
- Gene Information
- Related Products
Source : | Insect Cells |
Species : | H7N9 |
Tag : | C-His |
Protein length : | Met1-Val524 |
Form : | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.3-3 μg/mL for 1% GRBC. 2. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 3. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System. |
Molecular Mass : | The recombinant hemagglutinin of Influenza A virus (A/Shanghai/2/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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