Active Recombinant MERS-CoV Spike Protein (18-1296aa), C-His-tagged
Cat.No. : | S-13M |
Product Overview : | Recombinant MERS-CoV Spike (18-1296aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
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Description : | MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERSCoV. The rate of human transmission among household contacts of MERS patients has been approximately 5 % based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Source : | Baculovirus |
Species : | MERS-CoV |
Tag : | C-His |
Form : | Liquid |
Bio-activity : | Measured by its binding ability in a functional ELISA with Human DPPIV/CD26. |
Molecular Mass : | 141.6 kDa(1285 aa) |
Protein Length : | 18-1296 aa |
AA Sequence : | YVDVGPDSVKSACIEVDIQQTFFDK TWPRPIDVSKADGIIYPQGRTYSNI TITYQGLFPYQGDHGDMYVYSAGHA TGTTPQKLFVANYSQDVKQFANGFV VRIGAAANSTGTVIISPSTSATIRK IYPAFMLGSSVGNFSDGKMGRFFNH TLVLLPDGCGTLLRAFYCILEPRSG NHCPAGNSYTSFATYHTPATDCSDG NYNRNASLNSFKEYFNLRNCTFMYT YNITEDEILEWFGITQTAQGVHLFS SRYVDLYGGNMFQFATLPVYDTIKY YSIIPHSIRSIQSDRKAWAAFYVYK LQPLTFLLDFSVDGYIRRAIDCGFN DLSQLHCSYESFDVESGVYSVSSFE AKPSGSVVEQAEGVECDFSPLLSGT PPQVYNFKRLVFTNCNYNLTKLLSL FSVNDFTCSQISPAAIASNCYSSLI LDYFSYPLSMKSDLSVSSAGPISQF NYKQSFSNPTCLILATVPHNLTTIT KPLKYSYINKCSRLLSDDRTEVPQL VNANQYSPCVSIVPSTVWEDGDYYR KQLSPLEGGGWLVASGSTVAMTEQL QMGFGITVQYGTDTNSVCPKLEFAN DTKIASQLGNCVEYSLYGVSGRGVF QNCTAVGVRQQRFVYDAYQNLVGYY SDDGNYYCLRACVSVPVSVIYDKET KTHATLFGSVACEHISSTMSQYSRS TRSMLKRRDSTYGPLQTPVGCVLGL VNSSLFVEDCKLPLGQSLCALPDTP STLTPRSVRSVPGEMRLASIAFNHP IQVDQLNSSYFKLSIPTNFSFGVTQ EYIQTTIQKVTVDCKQYVCNGFQKC EQLLREYGQFCSKINQALHGANLRQ DDSVRNLFASVKSSQSSPIIPGFGG DFNLTLLEPVSISTGSRSARSAIED LLFDKVTIADPGYMQGYDDCMQQGP ASARDLICAQYVAGYKVLPPLMDVN MEAAYTSSLLGSIAGVGWTAGLSSF AAIPFAQSIFYRLNGVGITQQVLSE NQKLIANKFNQALGAMQTGFTTTNE AFQKVQDAVNNNAQALSKLASELSN TFGAISASIGDIIQRLDVLEQDAQI DRLINGRLTTLNAFVAQQLVRSESA ALSAQLAKDKVNECVKAQSKRSGFC GQGTHIVSFVVNAPNGLYFMHVGYY PSNHIEVVSAYGLCDAANPTNCIAP VNGYFIKTNNTRIVDEWSYTGSSFY APEPITSLNTKYVAPQVTYQNISTN LPPPLLGNSTGIDFQDELDEFFKNV STSIPNFGSLTQINTTLLDLTYEML SLQQVVKALNESYIDLKELGNYTYY NKWP |
Endotoxin : | < 1 EU/μg of protein (determined by LAL method) |
Purity : | > 85% by SDS-PAGE |
Applications : | SDS-PAGE, Bioactivity |
Notes : | For research use only. This product is not intended or approved for human, diagnostics or veterinary use. |
Storage : | Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.25 mg/mL (determined by Bradford assay) |
Storage Buffer : | Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Gene Name : | S spike protein [ Middle East respiratory syndrome-related coronavirus ] |
Official Symbol : | S |
Synonyms : | S; spike protein; surface glycoprotein |
Gene ID : | 14254594 |
Protein Refseq : | YP_009047204 |
UniProt ID : | K0BRG7 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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