Active Recombinant Mouse Alcam Protein, hIgG/His-tagged

• Cat.No.: Alcam-01M
• Product Overview: Recombinant mouse ALCAM (28-527aa), fused to hIgG-His-tag at C-terminus, was expressed in Insect cell and purified by using conventional chromatography techniques.

Specification

• Description: Predicted to enable identical protein binding activity. Acts upstream of or within motor neuron axon guidance. Located in axon; external side of plasma membrane; and neuronal cell body. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; sensory organ; and vibrissa.
• Source: Insect cells
• Species: Mouse
• Tag: hIgG/His
• Form: Liquid
• Bio-activity: Measured by its ability to block the adhesion of Jurkat human acute T cell leukemia cells to immobilized Recombinant Human CD6. When cells are added to 1 μg/mL of mouse ALCAM binded to CD6, This effect is more to 50%.
• Molecular Mass: 83.1 kDa (739aa)
• Protein length: 28-527aa
• AA Sequence: WYTVNSAYGDTIVMPCRLDVPQNLM FGKWKYEKPDGSPVFIAFRSSTKKS VQYDDVPEYKDRLSLSENYTLSIAN AKISDEKRFVCMLVTEDNVFEAPTL VKVFKQPSKPEIVNKAPFLETDQLK KLGDCISRDSYPDGNITWYRNGKVL QPVEGEVAILFKKEIDPGTQLYTVT SSLEYKTTRSDIQMPFTCSVTYYGP SGQKTIYSEQEIFDIYYPTEQVTIQ VLPPKNAIKEGDNITLQCLGNGNPP PEEFMFYLPGQPEGIRSSNTYTLTD VRRNATGDYKCSLIDKRNMAASTTI TVHYLDLSLNPSGEVTKQIGDTLPV SCTISASRNATVVWMKDNIRLRSSP SFSSLHYQDAGNYVCETALQEVEGL KKRESLTLIVEGKPQIKMTKKTDPS GLSKTIICHVEGFPKPAIHWTITGS GSVINQTEESPYINGRYYSKIIISP EENVTLTCTAENQLERTVNSLNVSA ISIPEHDEADDISDENREKVNDQAK
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol

Gene Information

• Gene Name: Alcam activated leukocyte cell adhesion molecule [ Mus musculus (house mouse) ]
• Official Symbol: Alcam
• Synonyms: Alcam; activated leukocyte cell adhesion molecule; BEN; SC1; MuSC; CD166; AI853494; DM-GRASP; CD166 antigen
• Gene ID: 11658
• mRNA Refseq: NM_009655
• Protein Refseq: NP_033785
• UniProt ID: Q61490