Recombinant Mouse Cd274 protein(Met1-Thr238), His-tagged

• Cat.No.: Cd274-2275M
• Product Overview: Recombinant Mouse CD274 (NP_068693.1)(Met 1-Thr 238) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Thr238
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-L1 at 1 μg/ml (100 μl/well) can bind mouse PD1 with a linear range of 6.25-400 ng/ml.
• Molecular Mass: The secreted recombinant mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, it migrates as an approximately 43-55 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus ]
• Official Symbol: Cd274
• Synonyms: CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693