Active Recombinant Mouse Chil3 Protein, His-tagged

• Cat.No.: Chil3-627M
• Product Overview: Recombinant Mouse Chil3 (Accession # NP_034022) Met1-Tyr398, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Tyr22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells.When 5 x 10^4 cells/well are added to Recombinant Mouse Chitinase 3-like 3/ECF-L coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is typically 0.3 - 1.2 µg/mL.
• Molecular Mass: Recombinant Mouse Chil3, His-tagged has a calculated MW of 43 kDa. In SDS-PAGE migrates as 43 kDa, reducing conditions.
• Protein length: Met1-Tyr398
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Chil3 chitinase-like 3 [ Mus musculus (house mouse) ]
• Official Symbol: Chil3
• Synonyms: Ym1; ECF-L; Chi3l3; AI505981; chitinase-like protein 3; chitinase-like protein 3; beta-N-acetylhexosaminidase Ym1; chitinase 3-like 3; chitinase-3-like protein 3; eosinophil chemotactic cytokine; eosinophil chemotactic factor-L; secreted protein Ym1
• Gene ID: 12655
• mRNA Refseq: NM_009892.2
• Protein Refseq: NP_034022.2
• Chromosome Location: 3 F2.2; 3 46.49 cM