Active Recombinant Mouse Dsg2, Fc-tagged

• Cat.No.: Dsg2-41M
• Product Overview: Recombinant Mouse Dsg2 was expressed in Mouse NSO Cells.

Specification

• Description: Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene.
• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Leu29 & Ala55
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BUD?8 human fibroblast cells.When 3 x 10^4 cells are added to Recombinant Mouse Desmoglein?2 Fc Chimera coated plates (10 μg/mL, 100 μL/well), approximately 30%?60% will adhere after 1 hour at 37 °C.Optimal dilutions should be determinded by each laboratory for each application.
• Molecular Mass: Predicted Molecular Mass: 92.9 kDa (monomer)SDS-PAGE: 100-120 kDa, reducing conditions
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 400 μg/mL in PBS.

Gene Information

• Gene Name: Dsg2 desmoglein 2 [ Mus musculus (house mouse) ]
• Official Symbol: Dsg2
• Synonyms: Dsg2; AA408168; D18Ertd293e; desmoglein 2; desmoglein-2
• Gene ID: 13511
• mRNA Refseq: NM_007883
• Protein Refseq: NP_031909
• UniProt ID: O55111
• Chromosome Location: 18 A2; 18 11.42 cM
• Pathway: Apoptosis; Apoptotic cleavage of cell adhesion proteins; Apoptotic execution phase
• Function: calcium ion binding; metal ion binding

Reviews

05/05/2021

Unmatched product superiority, professional customer service, and delivery that exceeded expectations.

02/01/2019

A truly delightful buying experience.

07/17/2018

This lab product excels in quality, backed by a customer service team that is not only friendly but also knowledgeable.

Q&As

How does Desmoglein-2 contribute to wound healing and tissue repair, and are there therapeutic implications for manipulating its activity in these processes?

Desmoglein-2 plays a role in wound healing and tissue repair by facilitating cell adhesion and migration. Manipulating its activity may have therapeutic implications for enhancing these processes.

How is the expression of Desmoglein-2 regulated, and what are the consequences of its dysregulation in the context of autoimmune diseases and skin disorders?

Desmoglein-2 expression is regulated at transcriptional and post-translational levels. Dysregulation is linked to autoimmune diseases like pemphigus, where autoantibodies target Desmoglein-2, leading to skin blistering.

Can you elaborate on the molecular structure of Desmoglein-2 and its interactions within desmosomal complexes?

Desmoglein-2 has a unique molecular structure with extracellular domains facilitating cell-cell adhesion. It interacts with other desmosomal components, such as Desmocollins and Plakoglobin, forming strong intercellular junctions.

How does Desmoglein-2 contribute to the regulation of cell signaling pathways, and are there specific pathways influenced by its interactions with other cellular components?

Desmoglein-2 participates in cell signaling, impacting pathways involved in cell adhesion, migration, and survival. Its interactions with other desmosomal components modulate signaling cascades essential for cellular function.

Can you discuss the role of Desmoglein-2 in epithelial-mesenchymal transition (EMT) and its implications for cancer invasion and metastasis?

Desmoglein-2 influences EMT, a process crucial for cancer invasion and metastasis. Changes in Desmoglein-2 expression and localization contribute to alterations in cell adhesion dynamics during EMT.