Active Recombinant Mouse Epo Protein, His-tagged

• Cat.No.: Epo-022M
• Product Overview: Recombinant mouse EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Insect cells
• Species: Mouse
• Tag: His
• Form: Liquid
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 2 ng/mL.
• Molecular Mass: 19.8 kDa
• AA Sequence: MAPPRLICDSRVLERYILEAKEAEN VTMGCAEGPRLSENITVPDTKVNFY AWKRMEVEEQAIEVWQGLSLLSEAI LQAQALLANSSQPPETLQLHIDKAI SGLRSLTSLLRVLGAQKELMSPPDT TPPAPLRTLTVDTFCKLFRVYANFL RGKLKLYTGEVCRRGDR
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -101 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Epo erythropoietin [ Mus musculus (house mouse) ]
• Official Symbol: Epo
• Synonyms: Epo; erythropoietin; erythropoietin
• Gene ID: 13856
• mRNA Refseq: NM_007942
• Protein Refseq: NP_031968
• UniProt ID: P07321