Active Recombinant Mouse FCGR2B protein, Avi-His-tagged, Biotinylated

• Cat.No.: FCGR2B-1441M
• Product Overview: Biotinylated Recombinant Mouse FCGR2B protein(Thr40-Arg217), fused with Avi and His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Avi-His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Loaded Biotinylated Mouse CD32b, Avitag,His Tag on SA Biosensor, can bind MabThera (Rituximab) with an affinity constant of 2.5 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 24.0 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Thr40-Arg217
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgr2b Fc receptor, IgG, low affinity IIb [ Mus musculus ]
• Official Symbol: FCGR2B
• Synonyms: FCGR2B; Fc receptor, IgG, low affinity IIb; low affinity immunoglobulin gamma Fc region receptor II-b; fcRII; FcgammaRIIB; Fc-gamma-RII; fc-gamma RII; Fc gamma RIIB; fc-gamma-RIIB; fc gamma receptor IIB; lymphocyte antigen 17; igG Fc receptor II beta; low affinity immunoglobulin gamma Fc region receptor II; CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik
• Gene ID: 14130
• mRNA Refseq: NM_001077189
• Protein Refseq: NP_001070657